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  • Featured Product
  • KD/KO Validated

UNC45A Polyclonal antibody

UNC45A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15479-1-AP

Synonyms

GC UNC45, GCUNC 45, GCUNC45, IRO039700, Protein unc 45 homolog A, SMAP 1, SMAP1, unc 45 homolog A (C. elegans), Unc 45A, UNC45A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UNC45A Polyclonal antibody (15479-1-AP)

    WB analysis of HeLa using 15479-1-AP

    WB result of UNC45A antibody (15479-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC45A transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using UNC45A Polyclonal antibody (15479-1-AP)

    WB analysis using 15479-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15479-1-AP (UNC45A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using UNC45A Polyclonal antibody (15479-1-AP)

    IP experiment of HeLa using 15479-1-AP

    IP result of anti-UNC45A (IP:15479-1-AP, 4ug; Detection:15479-1-AP 1:300) with HeLa cells lysate 1600 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using UNC45A Polyclonal antibody (15479-1-AP)

    IP experiment of HeLa using 15479-1-AP

    IP result of anti-UNC45A (IP:15479-1-AP, 4ug; Detection:15479-1-AP 1:1000) with HeLa cells lysate 1400ug.

  • Immunohistochemistry (IHC) staining of human cervix tissue using UNC45A Polyclonal antibody (15479-1-AP)

    IHC staining of human cervix using 15479-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 15479-1-AP (UNC45A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using UNC45A Polyclonal antibody (15479-1-AP)

    IHC staining of human cervix using 15479-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 15479-1-AP (UNC45A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UNC45A Polyclonal antibody (15479-1-AP)

    IF Staining of HepG2 using 15479-1-AP

    Immunofluorescent analysis of HepG2 cells, using UNC45A antibody 15479-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15479-1-AP targets UNC45A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UNC45A fusion protein Ag7790
Full Name unc-45 homolog A (C. elegans)
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC006214
Gene Symbol UNC45A
Gene ID (NCBI) 55898
RRIDAB_2212736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UNC45A antibody 15479-1-APDownload protocol
IHC protocol for UNC45A antibody 15479-1-APDownload protocol
IF protocol for UNC45A antibody 15479-1-APDownload protocol
IP protocol for UNC45A antibody 15479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.

Authors - Judd F Hultquist
  • KO Validated
View Article
fishELISA

Food Res Int

Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).

Authors - Lihong Ge
View Article
humanIHC

Mol Cancer Res

PMID: 30322860

Authors - Ashley Mooneyham
View Article
humanWB,IF

Cancer Biol Ther

UNC-45A is preferentially expressed in epithelial cells and binds to and co-localizes with interphase MTs.

Authors - Juri Habicht
View Article
humanWB,IP

J Biol Chem

Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors

Authors - Ya-Juan Wang
View Article