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UPK2 Polyclonal antibody

UPK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21149-1-AP

Synonyms

UP2, UPII, upk 2, UPK2, uroplakin 2, Uroplakin II

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using UPK2 Polyclonal antibody (21149-1-AP)

    WB analysis of mouse testis using 21149-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21149-1-AP (UPK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UPK2 Polyclonal antibody (21149-1-AP)

    WB analysis of mouse kidney using 21149-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21149-1-AP (UPK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human bladder tissue using UPK2 Polyclonal antibody (21149-1-AP)

    IHC staining of human bladder using 21149-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 21149-1-AP (UPK2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using UPK2 Polyclonal antibody (21149-1-AP)

    IHC staining of human bladder using 21149-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 21149-1-AP (UPK2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using UPK2 Polyclonal antibody (21149-1-AP)

    IHC staining of human bladder using 21149-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 21149-1-AP (UPK2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using UPK2 Polyclonal antibody (21149-1-AP)

    IHC staining of human bladder using 21149-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 21149-1-AP (UPK2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, mouse kidney tissue
Positive IHC detected inhuman bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21149-1-AP is UPK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPK2 fusion protein Ag15694
Full Name uroplakin 2
Calculated Molecular Weight 184 aa, 19 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC113900
Gene Symbol UPK2
Gene ID (NCBI) 7379
RRIDAB_10755290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-2 (UPK2) is a single-pass type I membrane protein. UPK2 may play an important role in regulating the assembly of the AUM.

Protocols

Product Specific Protocols
WB protocol for UPK2 antibody 21149-1-APDownload protocol
IHC protocol for UPK2 antibody 21149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biofabrication

Construction of a three-dimensional urothelium on-chip with barrier function based on urinary flow microenvironment

Authors - Changhao Hou
View Article
humanIF

Sci Rep

Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder.

Authors - Yuta Inoue
View Article
ratWB,IHC

Int J Mol Sci

Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2-Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat

Authors - Hidenori Akaihata
View Article