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UROS Polyclonal antibody

UROS Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14913-1-AP

Synonyms

UROIIIS, uroporphyrinogen III synthase, UROS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UROS Polyclonal antibody (14913-1-AP)

    WB analysis using 14913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UROS Polyclonal antibody (14913-1-AP)

    WB analysis of K-562 using 14913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UROS Polyclonal antibody (14913-1-AP)

    WB analysis of K-562 using 14913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using UROS Polyclonal antibody (14913-1-AP)

    IP experiment of K-562 using 14913-1-AP

    IP result of anti-UROS (IP:14913-1-AP, 4ug; Detection:14913-1-AP 1:500) with K-562 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using UROS Polyclonal antibody (14913-1-AP)

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using UROS Polyclonal antibody (14913-1-AP)

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UROS Polyclonal antibody (14913-1-AP)

    IF Staining of MCF-7 using 14913-1-AP

    Immunofluorescent analysis of MCF-7 cells, using UROS antibody 14913-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14913-1-AP targets UROS in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UROS fusion protein Ag6711
Full Name uroporphyrinogen III synthase
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC002573
Gene Symbol UROS
Gene ID (NCBI) 7390
RRIDAB_2213824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UROS antibody 14913-1-APDownload protocol
IHC protocol for UROS antibody 14913-1-APDownload protocol
IF protocol for UROS antibody 14913-1-APDownload protocol
IP protocol for UROS antibody 14913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols