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- KD/KO Validated
USP14 Polyclonal antibody
USP14 Polyclonal Antibody for WB, IP, IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14517-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, mouse heart tissue, HEK-293T cells, HEK-293 cells, SKOV-3 cells, Neuro-2a cells |
| Positive IP detected in | mouse liver tissue |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:18000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 14 publications below |
| IHC | See 4 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
14517-1-AP targets USP14 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP14 fusion protein Ag5979 |
| Full Name | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 52-60 kDa |
| GenBank Accession Number | BC003556 |
| Gene Symbol | USP14 |
| Gene ID (NCBI) | 9097 |
| RRID | AB_2257124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP14 antibody 14517-1-AP | Download protocol |
| IF protocol for USP14 antibody 14517-1-AP | Download protocol |
| IP protocol for USP14 antibody 14517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination. | ||
Autophagy Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.
| ||
Cell Prolif SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway. | ||
Front Pharmacol SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis. | ||
J Biol Chem The USP14-NLRC5 pathway inhibits titanium particle-induced osteolysis in mice by suppressing NF-κB and PI3K/AKT activities. | ||
