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UTP3 Polyclonal antibody

UTP3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18886-1-AP

Synonyms

CRL1, CRLZ1, Disrupter of silencing SAS10, SAS10, UTP3, UTP3 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using UTP3 Polyclonal antibody (18886-1-AP)

    WB analysis of A549 using 18886-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using UTP3 Polyclonal antibody (18886-1-AP)

    WB analysis of mouse thymus using 18886-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using UTP3 Polyclonal antibody (18886-1-AP)

    WB analysis of human brain using 18886-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UTP3 Polyclonal antibody (18886-1-AP)

    WB analysis of HeLa using 18886-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UTP3 Polyclonal antibody (18886-1-AP)

    WB analysis of HeLa using 18886-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UTP3 Polyclonal antibody (18886-1-AP)

    IF Staining of HepG2 using 18886-1-AP

    Immunofluorescent analysis of HepG2 cells, using UTP3 antibody 18886-1-AP at 1:50dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UTP3 Polyclonal antibody (18886-1-AP)

    IF Staining of HeLa using 18886-1-AP

    Immunofluorescent analysis of Hela cells, using UTP3 antibody 18886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, human brain tissue, mouse thymus tissue
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18886-1-AP targets UTP3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UTP3 fusion protein Ag13454
Full Name UTP3, small subunit (SSU) processome component, homolog (S. cerevisiae)
Calculated Molecular Weight 479 aa, 55 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC004546
Gene Symbol UTP3
Gene ID (NCBI) 57050
RRIDAB_10597076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UTP3 antibody 18886-1-APDownload protocol
IF protocol for UTP3 antibody 18886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols