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Ubiquilin 1 Polyclonal antibody

Ubiquilin 1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22126-1-AP

Synonyms

DA41, DSK2, hPLIC 1, PLIC 1, PLIC1, ubiquilin 1, UBQLN1, XDRP1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    WB analysis of rat brain using 22126-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    WB analysis of rat liver using 22126-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    WB analysis of Transfected HEK-293 using 22126-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    WB analysis of rat heart using 22126-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    WB analysis of rat liver using 22126-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    WB analysis of L02 using 22126-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ubiquilin 1 Polyclonal antibody (22126-1-AP)

    IF Staining of HeLa using 22126-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ubiquilin 1 antibody (22126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inrat brain tissue, L02 cells, rat heart tissue, rat liver tissue, Transfected HEK-293 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22126-1-AP targets Ubiquilin 1 in WB, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ubiquilin 1 fusion protein Ag17530
Full Name ubiquilin 1
Calculated Molecular Weight 589 aa, 63 kDa
Observed Molecular Weight 63-71 kDa
GenBank Accession NumberBC010066
Gene Symbol UBQLN1
Gene ID (NCBI) 29979
RRIDAB_2879000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.

Protocols

Product Specific Protocols
WB protocol for Ubiquilin 1 antibody 22126-1-APDownload protocol
IF protocol for Ubiquilin 1 antibody 22126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols