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VANGL2 Monoclonal antibody

VANGL2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B2C1

Cat no : 67273-1-Ig

Synonyms

KIAA1215, Loop tail protein 1 homolog, LPP1, LTAP, STB1, STBM, STBM1, Strabismus 1, Van Gogh like protein 2, Vang like protein 2, VANGL2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using VANGL2 Monoclonal antibody (67273-1-Ig)

    WB analysis of MCF-7 using 67273-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using VANGL2 Monoclonal antibody (67273-1-Ig)

    WB analysis of HepG2 using 67273-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VANGL2 Monoclonal antibody (67273-1-Ig)

    WB analysis of HeLa using 67273-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67273-1-Ig targets VANGL2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhamster
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen VANGL2 fusion protein Ag16980
Full Name vang-like 2 (van gogh, Drosophila)
Calculated Molecular Weight 521 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC103920
Gene Symbol VANGL2
Gene ID (NCBI) 57216
RRIDAB_2882542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.

Protocols

Product Specific Protocols
WB protocol for VANGL2 antibody 67273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Biochim Biophys Acta Gene Regul Mech

MicroRNA-27a-3p targeting Vangl1 and Vangl2 inhibits cell proliferation in mouse granulosa cells

Authors - Hu Tao
  • KD Validated
View Article