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VAV2 Polyclonal antibody

VAV2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21924-1-AP

Synonyms

VAV 2, VAV2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAV2 Polyclonal antibody (21924-1-AP)

    WB analysis using 21924-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VAV2 Polyclonal antibody (21924-1-AP)

    WB analysis using 21924-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using VAV2 Polyclonal antibody (21924-1-AP)

    WB analysis of HEK-293 using 21924-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VAV2 Polyclonal antibody (21924-1-AP)

    WB analysis of mouse brain using 21924-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using VAV2 Polyclonal antibody (21924-1-AP)

    WB analysis of A431 using 21924-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using VAV2 Polyclonal antibody (21924-1-AP)

    WB analysis of NIH/3T3 using 21924-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using VAV2 Polyclonal antibody (21924-1-AP)

    IP experiment of HeLa using 21924-1-AP

    IP result of anti-VAV2 (IP:21924-1-AP, 4ug; Detection:21924-1-AP 1:2000) with HeLa cells lysate 1520 ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using VAV2 Polyclonal antibody (21924-1-AP)

    IHC staining of human pancreas using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using VAV2 Polyclonal antibody (21924-1-AP)

    IHC staining of human pancreas using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VAV2 Polyclonal antibody (21924-1-AP)

    IHC staining of human kidney using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VAV2 Polyclonal antibody (21924-1-AP)

    IHC staining of human kidney using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VAV2 Polyclonal antibody (21924-1-AP)

    IF Staining of HeLa using 21924-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21924-1-AP (VAV2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, A431 cells, NIH/3T3 cells, HeLa cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21924-1-AP targets VAV2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAV2 fusion protein Ag16458
Full Name vav 2 guanine nucleotide exchange factor
Calculated Molecular Weight 839 aa, 97 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC132967
Gene Symbol VAV2
Gene ID (NCBI) 7410
RRIDAB_11182283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VAV2 antibody 21924-1-APDownload protocol
IHC protocol for VAV2 antibody 21924-1-APDownload protocol
IF protocol for VAV2 antibody 21924-1-APDownload protocol
IP protocol for VAV2 antibody 21924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Mesenchymal stem cells promote endothelial progenitor cell migration, vascularization, and bone repair in tissue-engineered constructs via activating CXCR2-Src-PKL/Vav2-Rac1.

Authors - Zhilin Li
View Article