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VAV2 Monoclonal antibody

VAV2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F11B5

Cat no : 67108-1-Ig

Synonyms

VAV 2, VAV2

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of fetal human brain using 67108-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of A431 using 67108-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of HeLa using 67108-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of HEK-293 using 67108-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of pig brain using 67108-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of rat brain using 67108-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VAV2 Monoclonal antibody (67108-1-Ig)

    WB analysis of mouse brain using 67108-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using VAV2 Monoclonal antibody (67108-1-Ig)

    IF Staining of A431 using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using VAV2 Monoclonal antibody (67108-1-Ig)

    IF Staining of HUVEC using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VAV2 Monoclonal antibody (67108-1-Ig)

    IF Staining of HeLa using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected infetal human brain tissue, A431 cells, HeLa cells, HEK-293 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF detected inA431 cells, HeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67108-1-Ig targets VAV2 in WB, IF, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VAV2 fusion protein Ag17988
Full Name vav 2 guanine nucleotide exchange factor
Calculated Molecular Weight 839 aa, 97 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC132967
Gene Symbol VAV2
Gene ID (NCBI) 7410
RRIDAB_2882412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VAV2 antibody 67108-1-IgDownload protocol
IF protocol for VAV2 antibody 67108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols