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KD/KO Validated

VPS37A Polyclonal antibody

Name: VPS37A Polyclonal antibody
Brand: Proteintech
SKU: 11870-1-AP

VPS37A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11870-1-AP

Synonyms

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

Validation Data Gallery

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AllWBIHCIF

WB analysis using 11870-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11870-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11870-1-AP
WB result of VPS37A antibody (11870-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS37A transfected MCF-7 cells.
WB analysis of MCF-7 using 11870-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11870-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11870-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11870-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11870-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 11870-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 11870-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 40x lens).
IF Staining of human liver using 11870-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VPS37A antibody (11870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11870-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	human liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 12 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

11870-1-AP targets VPS37A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VPS37A fusion protein Ag2453
Full Name	vacuolar protein sorting 37 homolog A (S. cerevisiae)
Calculated Molecular Weight	44 kDa
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC022363
Gene Symbol	VPS37A
Gene ID (NCBI)	137492
RRID	AB_2215230
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Protocols

Product Specific Protocols
WB protocol for VPS37A antibody 11870-1-AP	Download protocol
IHC protocol for VPS37A antibody 11870-1-AP	Download protocol
IF protocol for VPS37A antibody 11870-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Autophagy Removal of hypersignaling endosomes by simaphagy Authors - Simona M Migliano KO Validated View Article
human	WB	Curr Biol UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Authors - Stefani Flavia F View Article
human	WB	Structure The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain. Authors - Monica Agromayor View Article
human	WB,IHC	Cell Death Dis HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer. Authors - Feifei Chen KD Validated View Article
human	WB	J Cell Sci The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex. Authors - Lydia Wunderley View Article
human	WB	J Med Genet A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis. Authors - Zivony-Elboum Yifat Y View Article

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Research Area

VPS37A Polyclonal antibody

VPS37A Polyclonal Antibody for WB, IF, IHC, ELISA

Validation Data Gallery

WB analysis using 11870-1-AP

WB analysis of human liver using 11870-1-AP

WB analysis of MCF-7 using 11870-1-AP

WB analysis of MCF-7 using 11870-1-AP

WB analysis of mouse liver using 11870-1-AP

WB analysis of HeLa using 11870-1-AP

WB analysis of MCF-7 using 11870-1-AP

WB analysis of HeLa using 11870-1-AP

IHC staining of human kidney using 11870-1-AP

IHC staining of human kidney using 11870-1-AP

IF Staining of human liver using 11870-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Removal of hypersignaling endosomes by simaphagy

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.

HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer.

The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex.

A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis.