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VPS37A Monoclonal antibody

VPS37A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9A4

Cat no : 66327-1-Ig

Synonyms

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

Validation Data Gallery

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  • Western Blot (WB) analysis of SW 1990 cells using VPS37A Monoclonal antibody (66327-1-Ig)

    WB analysis of SW 1990 using 66327-1-Ig

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using VPS37A Monoclonal antibody (66327-1-Ig)

    WB analysis of MCF-7 using 66327-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human kidney using 66327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human kidney using 66327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human ovary tumor using 66327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human ovary tumor using 66327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using VPS37A Monoclonal antibody (66327-1-Ig)

    IF Staining of MCF-7 using 66327-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inSW 1990 cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66327-1-Ig targets VPS37A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS37A fusion protein Ag19108
Full Name vacuolar protein sorting 37 homolog A (S. cerevisiae)
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC022363
Gene Symbol VPS37A
Gene ID (NCBI) 137492
RRIDAB_2881708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Protocols

Product Specific Protocols
WB protocol for VPS37A antibody 66327-1-IgDownload protocol
IHC protocol for VPS37A antibody 66327-1-IgDownload protocol
IF protocol for VPS37A antibody 66327-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Endocrinol (Lausanne)

Stratification of diabetic kidney diseases via data-independent acquisition proteomics–based analysis of human kidney tissue specimens

Authors - Qinghua Huang
View Article