• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

VPS8 Polyclonal antibody

VPS8 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15079-1-AP

Synonyms

KIAA0804, VPS8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using VPS8 Polyclonal antibody (15079-1-AP)

    WB analysis of human brain using 15079-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using VPS8 Polyclonal antibody (15079-1-AP)

    WB analysis of mouse liver using 15079-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using VPS8 Polyclonal antibody (15079-1-AP)

    WB analysis of human testis using 15079-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using VPS8 Polyclonal antibody (15079-1-AP)

    WB analysis of PC-3 using 15079-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VPS8 Polyclonal antibody (15079-1-AP)

    WB analysis of mouse brain using 15079-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse brain tissue using VPS8 Polyclonal antibody (15079-1-AP)

    WB analysis of mouse brain using 15079-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VPS8 Polyclonal antibody (15079-1-AP)

    IP experiment of mouse brain using 15079-1-AP

    IP result of anti-VPS8 (IP:15079-1-AP, 4ug; Detection:15079-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS8 Polyclonal antibody (15079-1-AP)

    IF Staining of HepG2 using 15079-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15079-1-AP (VPS8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, human testis tissue, mouse brain tissue, mouse liver tissue, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15079-1-AP targets VPS8 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS8 fusion protein Ag7151
Full Name vacuolar protein sorting 8 homolog (S. cerevisiae)
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 151 kDa
GenBank Accession NumberBC001001
Gene Symbol VPS8
Gene ID (NCBI) 23355
RRIDAB_2215543
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.

Protocols

Product Specific Protocols
WB protocol for VPS8 antibody 15079-1-APDownload protocol
IF protocol for VPS8 antibody 15079-1-APDownload protocol
IP protocol for VPS8 antibody 15079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang
  • KD Validated
View Article
humanWB, IP

Traffic

Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations.

Authors - Enrico Daniele Perini
View Article
humanWB

Biochem J

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

Authors - Morag R Hunter
View Article
humanWB

Sci Rep

HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis

Authors - Seigo Terawaki
  • KO Validated
View Article