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WDR16 Polyclonal antibody

WDR16 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12644-1-AP

Synonyms

WD repeat domain 16, WDR16, WDRPUH

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using WDR16 Polyclonal antibody (12644-1-AP)

    IHC staining of human testis using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR16 Polyclonal antibody (12644-1-AP)

    IHC staining of human testis using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WDR16 Polyclonal antibody (12644-1-AP)

    IHC staining of human liver cancer using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WDR16 Polyclonal antibody (12644-1-AP)

    IHC staining of human liver cancer using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WDR16 Polyclonal antibody (12644-1-AP)

    IF Staining of HeLa using 12644-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12644-1-AP (WDR16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12644-1-AP targets WDR16 in IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR16 fusion protein Ag3281
Full Name WD repeat domain 16
Calculated Molecular Weight 620 aa, 68 kDa
GenBank Accession NumberBC025392
Gene Symbol WDR16
Gene ID (NCBI) 146845
RRIDAB_2272921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for WDR16 antibody 12644-1-APDownload protocol
IF protocol for WDR16 antibody 12644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols