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WDR7 Polyclonal antibody

WDR7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24431-1-AP

Synonyms

KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR7 Polyclonal antibody (24431-1-AP)

    WB analysis using 24431-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24431-1-AP (WDR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using WDR7 Polyclonal antibody (24431-1-AP)

    IHC staining of human liver using 24431-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using WDR7 Polyclonal antibody (24431-1-AP)

    IHC staining of human liver using 24431-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR7 Polyclonal antibody (24431-1-AP)

    IHC staining of mouse testis using 24431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR7 Polyclonal antibody (24431-1-AP)

    IHC staining of mouse testis using 24431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 24431-1-AP is WDR7 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR7 fusion protein Ag19072
Full Name WD repeat domain 7
Calculated Molecular Weight 1490 aa, 164 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC131558
Gene Symbol WDR7
Gene ID (NCBI) 23335
RRIDAB_2879546
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR7, also named as KIAA0541 and TRAG, belongs to the WD-repeat protein family. WD repeats are areas with the repetitions of approximately 40 amino acids that have ends with trp-asp and gly-his amino acids (GH-WD) which may help to form a multi-protein complex. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle (PMID: 32128092). WDR7 has 2 isoforms with the molecular mass of 160 and 164 kDa. Sometimes lower molecular weight around 150 kDa can also be observed, which may be a modified variant of WDR7.

Protocols

Product Specific Protocols
WB protocol for WDR7 antibody 24431-1-APDownload protocol
IHC protocol for WDR7 antibody 24431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Biol Chem

The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells.

Authors - Ellen Crummy
View Article