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KD/KO Validated

WRAP53 Polyclonal antibody

Name: WRAP53 Polyclonal antibody
Brand: Proteintech
SKU: 14761-1-AP

WRAP53 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14761-1-AP

Synonyms

TCAB1, WDR79, WRAP53

Validation Data Gallery

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AllWBIPIHC

WB analysis using 14761-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 14761-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14761-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 14761-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14761-1-AP
IP result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.
IHC staining of mouse testis using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 14761-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 14761-1-AP

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Tested Applications

Positive WB detected in	Raji cells, human kidney tissue, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

14761-1-AP targets WRAP53 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	WRAP53 fusion protein Ag6522
Full Name	WD repeat containing, antisense to TP53
Calculated Molecular Weight	59 kDa
Observed Molecular Weight	72-75 kDa
GenBank Accession Number	BC002336
Gene Symbol	WRAP53
Gene ID (NCBI)	55135
RRID	AB_1939806
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WRAP53 antibody 14761-1-AP	Download protocol
IHC protocol for WRAP53 antibody 14761-1-AP	Download protocol
IP protocol for WRAP53 antibody 14761-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Genes Dev The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. Authors - Sofia Henriksson View Article
human	WB	Nat Chem Biol Observation of processive telomerase catalysis using high-resolution optical tweezers. Authors - Eric M Patrick View Article
human	WB	Oncogenesis RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks. Authors - Qunsong Tan KD Validated View Article
	WB	Aging (Albany NY) Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism. Authors - Xing-Hua Pan View Article
human	WB,IF	RNA Biol Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response. Authors - Christos Coucoravas KD Validated View Article
human	WB	Cancer Cell Int Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells. Authors - Jing Niu View Article

WRAP53 Polyclonal antibody

WRAP53 Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 14761-1-AP

WB analysis of human kidney using 14761-1-AP

WB analysis of HeLa using 14761-1-AP

WB analysis of Raji using 14761-1-AP

IP experiment of HeLa using 14761-1-AP

IHC staining of mouse testis using 14761-1-AP

IHC staining of mouse testis using 14761-1-AP

IHC staining of mouse testis using 14761-1-AP

IHC staining of mouse testis using 14761-1-AP

IHC staining of human heart using 14761-1-AP

IHC staining of human heart using 14761-1-AP

IHC staining of human kidney using 14761-1-AP

IHC staining of human kidney using 14761-1-AP

IHC staining of human placenta using 14761-1-AP

IHC staining of human placenta using 14761-1-AP

IHC staining of human testis using 14761-1-AP

IHC staining of human testis using 14761-1-AP

IHC staining of human skin using 14761-1-AP

IHC staining of human skin using 14761-1-AP

IHC staining of human brain using 14761-1-AP

IHC staining of human brain using 14761-1-AP

IHC staining of human spleen using 14761-1-AP

IHC staining of human spleen using 14761-1-AP

IHC staining of human ovary using 14761-1-AP

IHC staining of human ovary using 14761-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.

Observation of processive telomerase catalysis using high-resolution optical tweezers.

RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.

Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.