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WRN Polyclonal antibody

WRN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25269-1-AP

Synonyms

DNA helicase, RecQ like type 3, Exonuclease WRN, RecQ protein like 2, RECQ3, RECQL2, RECQL3, WRN

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using WRN Polyclonal antibody (25269-1-AP)

    WB analysis of MCF-7 using 25269-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using WRN Polyclonal antibody (25269-1-AP)

    WB analysis of HEK-293 using 25269-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25269-1-AP targets WRN in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Werner syndrome, RecQ helicase-like
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_000553
Gene Symbol WRN
Gene ID (NCBI) 7486
RRIDAB_2879997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Werner syndrome ATP dependent helicase (WRN) ,also named as RECQ3 or RecQ protein like 2, is a 1432 amino acid protein, which contains one HRDC domain, one helicase C-terminal domain, one helicase ATP-binding domain and 3'-5' exonuclease domain. WRN localizes in the nucleoplasm and belongs to the helicase family. WRN plays a central role in the repair of methylation-induced DNA damage and has an helicase activity necessary to prevent dramatic telomere loss during DNA replication. WRN gene mutation has an association with Werner syndrome and colorectal cancer.

Protocols

Product Specific Protocols
WB protocol for WRN antibody 25269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols