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YRDC Polyclonal antibody

YRDC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26295-1-AP

Synonyms

DRIP3, hIRIP, IRIP, RP11 109P14.4, SUA5, YRDC

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using YRDC Polyclonal antibody (26295-1-AP)

    WB analysis of HEK-293T using 26295-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26295-1-AP (YRDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using YRDC Polyclonal antibody (26295-1-AP)

    WB analysis of BxPC-3 using 26295-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26295-1-AP (YRDC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using YRDC Polyclonal antibody (26295-1-AP)

    WB analysis of RAW 264.7 using 26295-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26295-1-AP (YRDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using YRDC Polyclonal antibody (26295-1-AP)

    IHC staining of human pancreas using 26295-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26295-1-AP (YRDC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using YRDC Polyclonal antibody (26295-1-AP)

    IHC staining of human pancreas using 26295-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26295-1-AP (YRDC Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293T cells, BxPC-3 cells, RAW 264.7 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26295-1-AP targets YRDC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YRDC fusion protein Ag23633
Full Name yrdC domain containing (E. coli)
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC080186
Gene Symbol YRDC
Gene ID (NCBI) 79693
RRIDAB_2880468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for YRDC antibody 26295-1-APDownload protocol
IHC protocol for YRDC antibody 26295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols