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ZBTB25 Polyclonal antibody

ZBTB25 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25631-1-AP

Synonyms

C14orf51, KUP, ZBTB25, Zinc finger protein 46, Zinc finger protein KUP, ZNF46

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZBTB25 Polyclonal antibody (25631-1-AP)

    WB analysis of mouse testis using 25631-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse spleen tissue using ZBTB25 Polyclonal antibody (25631-1-AP)

    WB analysis of mouse spleen using 25631-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse testis tissue using ZBTB25 Polyclonal antibody (25631-1-AP)

    WB analysis of mouse testis using 25631-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZBTB25 Polyclonal antibody (25631-1-AP)

    IF Staining of HepG2 using 25631-1-AP

    Immunofluorescent analysis of HepG2 cells using 25631-1-AP (ZBTB25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, mouse spleen tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25631-1-AP is ZBTB25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB25 fusion protein Ag22507
Full Name zinc finger and BTB domain containing 25
Calculated Molecular Weight 435 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC035804
Gene Symbol ZBTB25
Gene ID (NCBI) 7597
RRIDAB_2880171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZBTB25 antibody 25631-1-APDownload protocol
IF protocol for ZBTB25 antibody 25631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article