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ZC3H18 Polyclonal antibody

ZC3H18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25807-1-AP

Synonyms

NHN1, Nuclear protein NHN1, ZC3H18

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZC3H18 Polyclonal antibody (25807-1-AP)

    WB analysis of HepG2 using 25807-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25807-1-AP (ZC3H18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZC3H18 Polyclonal antibody (25807-1-AP)

    IP experiment of HeLa using 25807-1-AP

    IP result of anti-ZC3H18 (IP:25807-1-AP, 4ug; Detection:25807-1-AP 1:600) with HeLa cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZC3H18 Polyclonal antibody (25807-1-AP)

    IHC staining of human tonsillitis using 25807-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ZC3H18 Polyclonal antibody (25807-1-AP)

    IHC staining of human cervical cancer using 25807-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ZC3H18 Polyclonal antibody (25807-1-AP)

    IHC staining of human cervical cancer using 25807-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZC3H18 Polyclonal antibody (25807-1-AP)

    IHC staining of human tonsillitis using 25807-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25807-1-AP is ZC3H18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3H18 fusion protein Ag20481
Full Name zinc finger CCCH-type containing 18
Calculated Molecular Weight 953 aa, 106 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC050463
Gene Symbol ZC3H18
Gene ID (NCBI) 124245
RRIDAB_2721255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H18, also named as NHN1, has two CCCH zinc finger domains. It is not essential in bloodstream forms, but in an in vitro model of differentiation. ZC3H18 recruites the TREX protein for SEP1-mediated mRNA export. The gene ZC3H18 has some isoforms with calculated MW 160 kDa and 84 kDa. With phospho modification, the MW of ZC3H18 is about 150 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for ZC3H18 antibody 25807-1-APDownload protocol
IHC protocol for ZC3H18 antibody 25807-1-APDownload protocol
IP protocol for ZC3H18 antibody 25807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols