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ZC3HC1 Polyclonal antibody

ZC3HC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13148-1-AP

Synonyms

1110054L24Rik, hNIPA, HSPC216, NIPA, ZC3HC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZC3HC1 Polyclonal antibody (13148-1-AP)

    WB analysis using 13148-1-AP

    A431 cell lysates were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ZC3HC1 Polyclonal antibody (13148-1-AP)

    WB analysis of mouse skeletal muscle using 13148-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ZC3HC1 Polyclonal antibody (13148-1-AP)

    WB analysis of mouse heart using 13148-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ZC3HC1 Polyclonal antibody (13148-1-AP)

    WB analysis of mouse lung using 13148-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, mouse skeletal muscle tissue, mouse heart tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13148-1-AP targets ZC3HC1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3HC1 fusion protein Ag3744
Full Name zinc finger, C3HC-type containing 1
Calculated Molecular Weight 502 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC028917
Gene Symbol ZC3HC1
Gene ID (NCBI) 51530
RRIDAB_2217514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZC3HC1 antibody 13148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Authors - Hao-Yu Li
View Article