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ZDHHC9 Polyclonal antibody

ZDHHC9 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24046-1-AP

Synonyms

CGI 89, CXorf11, DHHC 9, DHHC9, MMSA 1, Palmitoyltransferase ZDHHC9, UNQ261/PRO298, ZDHHC10, ZDHHC9, Zinc finger protein 379, ZNF379, ZNF380

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis using 24046-1-AP

    Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of SH-SY5Y using 24046-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse liver using 24046-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse brain using 24046-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse skeletal muscle using 24046-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse brain using 24046-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IHC staining of human testis using 24046-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IHC staining of human liver using 24046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IHC staining of human testis using 24046-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IF Staining of SH-SY5Y using 24046-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse brain tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24046-1-AP targets ZDHHC9 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZDHHC9 fusion protein Ag20881
Full Name zinc finger, DHHC-type containing 9
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38-48 kDa
GenBank Accession NumberBC006200
Gene Symbol ZDHHC9
Gene ID (NCBI) 51114
RRIDAB_2879415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Protocols

Product Specific Protocols
WB protocol for ZDHHC9 antibody 24046-1-APDownload protocol
IHC protocol for ZDHHC9 antibody 24046-1-APDownload protocol
IF protocol for ZDHHC9 antibody 24046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Discov Oncol

ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance

Authors - Chao Xu
View Article