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ZER1 Polyclonal antibody

ZER1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16647-1-AP

Synonyms

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZER1 Polyclonal antibody (16647-1-AP)

    WB analysis using 16647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16647-1-AP (ZER1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using ZER1 Polyclonal antibody (16647-1-AP)

    WB analysis of rat testis using 16647-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16647-1-AP (ZER1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ZER1 Polyclonal antibody (16647-1-AP)

    IP experiment of mouse testis using 16647-1-AP

    IP result of anti-ZER1 (IP:16647-1-AP, 3ug; Detection:16647-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human ovary tissue using ZER1 Polyclonal antibody (16647-1-AP)

    IHC staining of human ovary using 16647-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZER1 Polyclonal antibody (16647-1-AP)

    IHC staining of human testis using 16647-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ZER1 Polyclonal antibody (16647-1-AP)

    IHC staining of human ovary using 16647-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZER1 Polyclonal antibody (16647-1-AP)

    IHC staining of human testis using 16647-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inPC-3 cells, rat testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16647-1-AP is ZER1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZER1 fusion protein Ag10031
Full Name zer-1 homolog (C. elegans)
Calculated Molecular Weight 766 aa, 88 kDa
Observed Molecular Weight 80-88 kDa
GenBank Accession NumberBC052563
Gene Symbol ZER1
Gene ID (NCBI) 10444
RRIDAB_2878294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZER1 antibody 16647-1-APDownload protocol
IHC protocol for ZER1 antibody 16647-1-APDownload protocol
IP protocol for ZER1 antibody 16647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols