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ZFP64 Polyclonal antibody

ZFP64 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, chIP, ELISA

Conjugate

Unconjugated

Cat no : 17187-1-AP

Synonyms

Zfp 64, ZFP64, Zinc finger protein 338, ZNF338

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ZFP64 Polyclonal antibody (17187-1-AP)

    WB analysis of mouse spleen using 17187-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZFP64 Polyclonal antibody (17187-1-AP)

    WB analysis of HEK-293 using 17187-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse spleen tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

ChIPSee 1 publications below

Product Information

17187-1-AP targets ZFP64 in WB, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFP64 fusion protein Ag10971
Full Name zinc finger protein 64 homolog (mouse)
Calculated Molecular Weight 681 aa, 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC041622
Gene Symbol ZFP64
Gene ID (NCBI) 55734
RRIDAB_2218826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.

Protocols

Product Specific Protocols
WB protocol for ZFP64 antibody 17187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchIP

Cancer Cell

A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.

Authors - Bin Lu
View Article