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ZNF101 Polyclonal antibody

ZNF101 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25599-1-AP

Synonyms

HZF12, zinc finger protein 101, Zinc finger protein HZF12, ZNF101

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF101 Polyclonal antibody (25599-1-AP)

    WB analysis of HepG2 using 25599-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25599-1-AP (ZNF101 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF101 Polyclonal antibody (25599-1-AP)

    WB analysis of HeLa using 25599-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25599-1-AP (ZNF101 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF101 Polyclonal antibody (25599-1-AP)

    IF Staining of HepG2 using 25599-1-AP

    Immunofluorescent analysis of HepG2 cells using 25599-1-AP (ZNF101 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25599-1-AP targets ZNF101 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF101 fusion protein Ag22390
Full Name zinc finger protein 101
Calculated Molecular Weight 436 aa, 50 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC119680
Gene Symbol ZNF101
Gene ID (NCBI) 94039
RRIDAB_2880152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF101 antibody 25599-1-APDownload protocol
IF protocol for ZNF101 antibody 25599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols