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ZNF140 Polyclonal antibody

ZNF140 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 24531-1-AP

Synonyms

pHZ 39, zinc finger protein 140, ZNF140

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using ZNF140 Polyclonal antibody (24531-1-AP)

    WB analysis of SKOV-3 using 24531-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF140 Polyclonal antibody (24531-1-AP)

    WB analysis of Jurkat using 24531-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF140 Polyclonal antibody (24531-1-AP)

    IF Staining of HepG2 using 24531-1-AP

    Immunofluorescent analysis of HepG2 cells using 24531-1-AP (ZNF140 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inSKOV-3 cells, Jurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24531-1-AP is ZNF140 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF140 fusion protein Ag21379
Full Name zinc finger protein 140
Calculated Molecular Weight 457 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC040561
Gene Symbol ZNF140
Gene ID (NCBI) 7699
RRIDAB_2879592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF140 antibody 24531-1-APDownload protocol
IF protocol for ZNF140 antibody 24531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols