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ZNF311 Polyclonal antibody

ZNF311 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 23243-1-AP

Synonyms

zf31, ZFP31, zinc finger protein 311, Zinc finger protein zfp 31, ZNF311

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using ZNF311 Polyclonal antibody (23243-1-AP)

    WB analysis of HL-60 using 23243-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 23243-1-AP (ZNF311 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ZNF311 Polyclonal antibody (23243-1-AP)

    WB analysis of HL-60 using 23243-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 23243-1-AP (ZNF311 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF311 Polyclonal antibody (23243-1-AP)

    IHC staining of human testis using 23243-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF311 Polyclonal antibody (23243-1-AP)

    IHC staining of human kidney using 23243-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF311 Polyclonal antibody (23243-1-AP)

    IHC staining of human kidney using 23243-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF311 Polyclonal antibody (23243-1-AP)

    IHC staining of human testis using 23243-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23243-1-AP is ZNF311 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF311 fusion protein Ag19624
Full Name zinc finger protein 311
Calculated Molecular Weight 666 aa, 76 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC136745
Gene Symbol ZNF311
Gene ID (NCBI) 282890
RRIDAB_11232217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 311 (ZNF311), also named Zinc finger protein zfp-31 (ZFP31), is involved in regulation of transcription. It belongs to the krueppel C2H2-type zinfinger protein family, and contains 14 C2H2-type zinc fingers and 1 KRAB domain.This antibody specifically reacts with ZNF311 protein.

Protocols

Product Specific Protocols
WB protocol for ZNF311 antibody 23243-1-APDownload protocol
IHC protocol for ZNF311 antibody 23243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols