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ZNF34 Polyclonal antibody

ZNF34 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20477-1-AP

Synonyms

KOX32, zinc finger protein 34, Zinc finger protein KOX32, ZNF34

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF34 Polyclonal antibody (20477-1-AP)

    WB analysis of HeLa using 20477-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF34 Polyclonal antibody (20477-1-AP)

    WB analysis of HepG2 using 20477-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF34 Polyclonal antibody (20477-1-AP)

    WB analysis of Jurkat using 20477-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF34 Polyclonal antibody (20477-1-AP)

    IHC staining of human brain using 20477-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20477-1-AP (ZNF34 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF34 Polyclonal antibody (20477-1-AP)

    IHC staining of human brain using 20477-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20477-1-AP (ZNF34 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20477-1-AP is ZNF34 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF34 fusion protein Ag14346
Full Name zinc finger protein 34
Calculated Molecular Weight539aa,62 kDa; 560aa,64 kDa
Observed Molecular Weight 62-68 kDa
GenBank Accession NumberBC004480
Gene Symbol ZNF34
Gene ID (NCBI) 80778
RRIDAB_10665367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF34, also named as KOX32, contains twelve C2H2-type zinc fingers and one KRAB domain. It belongs to the krueppel C2H2-type zinc-finger protein family. This protein may be involved in transcriptional regulation.

Protocols

Product Specific Protocols
WB protocol for ZNF34 antibody 20477-1-APDownload protocol
IHC protocol for ZNF34 antibody 20477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols