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ZNF497 Polyclonal antibody

ZNF497 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25178-1-AP

Synonyms

zinc finger protein 497, ZNF497

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZNF497 Polyclonal antibody (25178-1-AP)

    WB analysis of mouse testis using 25178-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25178-1-AP (ZNF497 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF497 Polyclonal antibody (25178-1-AP)

    IHC staining of human testis using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF497 Polyclonal antibody (25178-1-AP)

    IHC staining of human kidney using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF497 Polyclonal antibody (25178-1-AP)

    IHC staining of human testis using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF497 Polyclonal antibody (25178-1-AP)

    IHC staining of human kidney using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25178-1-AP is ZNF497 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF497 fusion protein Ag18114
Full Name zinc finger protein 497
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC118983
Gene Symbol ZNF497
Gene ID (NCBI) 162968
RRIDAB_2879944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF497 antibody 25178-1-APDownload protocol
IHC protocol for ZNF497 antibody 25178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols