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ZNF549 Polyclonal antibody

ZNF549 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25592-1-AP

Synonyms

zinc finger protein 549, ZNF549

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF549 Polyclonal antibody (25592-1-AP)

    WB analysis of SH-SY5Y using 25592-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25592-1-AP (ZNF549 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZNF549 Polyclonal antibody (25592-1-AP)

    WB analysis of SMMC-7721 using 25592-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25592-1-AP (ZNF549 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ZNF549 Polyclonal antibody (25592-1-AP)

    WB analysis of A549 using 25592-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25592-1-AP (ZNF549 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF549 Polyclonal antibody (25592-1-AP)

    WB analysis of HeLa using 25592-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25592-1-AP (ZNF549 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ZNF549 Polyclonal antibody (25592-1-AP)

    IF Staining of A549 using 25592-1-AP

    Immunofluorescent analysis of A549 cells using 25592-1-AP (ZNF549 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells, A549 cells, HeLa cells, SMMC-7721 cells
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25592-1-AP is ZNF549 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF549 fusion protein Ag22371
Full Name zinc finger protein 549
Calculated Molecular Weight 640 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC060863
Gene Symbol ZNF549
Gene ID (NCBI) 256051
RRIDAB_2880147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF549 antibody 25592-1-APDownload protocol
IF protocol for ZNF549 antibody 25592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols