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ZNF571 Polyclonal antibody

ZNF571 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25302-1-AP

Synonyms

HSPC059, zinc finger protein 571, ZNF571

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ZNF571 Polyclonal antibody (25302-1-AP)

    WB analysis of MCF-7 using 25302-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25302-1-AP (ZNF571 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF571 Polyclonal antibody (25302-1-AP)

    WB analysis of HeLa using 25302-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25302-1-AP (ZNF571 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ZNF571 Polyclonal antibody (25302-1-AP)

    IP experiment of MCF-7 using 25302-1-AP

    IP result of anti-ZNF571 (IP:25302-1-AP, 4ug; Detection:25302-1-AP 1:300) with MCF-7 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF571 Polyclonal antibody (25302-1-AP)

    IHC staining of human testis using 25302-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25302-1-AP (ZNF571 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF571 Polyclonal antibody (25302-1-AP)

    IHC staining of human testis using 25302-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25302-1-AP (ZNF571 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25302-1-AP is ZNF571 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF571 fusion protein Ag18151
Full Name zinc finger protein 571
Calculated Molecular Weight 609 aa, 71 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC114479
Gene Symbol ZNF571
Gene ID (NCBI) 51276
RRIDAB_2880019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF571 antibody 25302-1-APDownload protocol
IHC protocol for ZNF571 antibody 25302-1-APDownload protocol
IP protocol for ZNF571 antibody 25302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Daniel (Verified Customer) (10-26-2022)

This antibody cross-reacts with other protein and produces several non-specific bands. The band of interest is usually sandwiched between two non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:700
  • Cell Tissue Type: HeLa, MT4 cells
ZNF571 Antibody Western Blot validation (1:700 dilution) in HeLa, MT4 cells (Cat no:25302-1-AP)