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domain-I-of-FIZ-1 Polyclonal antibody

domain-I-of-FIZ-1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15826-1-AP

Synonyms

domain I of FIZ 1, domain II of FIZ1, FIZ1, FLT3 interacting zinc finger 1, Zinc finger protein 798, ZNF798

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    WB analysis of human kidney using 15826-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    WB analysis of Y79 using 15826-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    WB analysis of human kidney using 15826-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IHC staining of human kidney using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IHC staining of human skeletal muscle using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IHC staining of human skeletal muscle using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IHC staining of human kidney using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IHC staining of human kidney using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IHC staining of human kidney using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using domain-I-of-FIZ-1 Polyclonal antibody (15826-1-AP)

    IF Staining of HepG2 using 15826-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inhuman kidney tissue, Y79 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15826-1-AP targets domain-I-of-FIZ-1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name FLT3-interacting zinc finger 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_032836
Gene Symbol FIZ1
Gene ID (NCBI) 84922
RRIDAB_2102547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for domain-I-of-FIZ-1 antibody 15826-1-APDownload protocol
IHC protocol for domain-I-of-FIZ-1 antibody 15826-1-APDownload protocol
IF protocol for domain-I-of-FIZ-1 antibody 15826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols