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UFM1 Polyclonal antibody

UFM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15883-1-AP

Synonyms

BM 002, C13orf20, ubiquitin fold modifier 1, UFM1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of mouse lung using 15883-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis using 15883-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of HEK-293 using 15883-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of HepG2 using 15883-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of mouse liver using 15883-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UFM1 Polyclonal antibody (15883-1-AP)

    IHC staining of human lung cancer using 15883-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UFM1 Polyclonal antibody (15883-1-AP)

    IHC staining of human lung cancer using 15883-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHT-29 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse lung tissue, L02 cells, U-251 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15883-1-AP targets UFM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UFM1 fusion protein Ag8667
Full Name ubiquitin-fold modifier 1
Calculated Molecular Weight 85 aa, 9 kDa
Observed Molecular Weight 9-12 kDa
GenBank Accession NumberBC005193
Gene Symbol UFM1
Gene ID (NCBI) 51569
RRIDAB_2878195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UFM1 (Ubiquitin-fold modifier 1) is a ubiquitin-like protein covalently conjugated with intracellular proteins through UFMylation (modification by UFM1), a process similar to ubiquitylation (PMID: 38141606). UFM1 is conjugated to its target proteins by E1-like activating enzyme UBE1DC1 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation (PMID: 28234446; 33066455). At the molecular level, UFMylation is an important mediator of the protein function. Dysregulation of the UFM1 system, e.g., the knockout of UFMylation components, disturbs proteome homeostasis and triggers endoplasmic reticulum stress. Such changes are linked to developmental disorders, tumorigenesis, tissue injury, inflammation, and several hereditary neurological syndromes (PMID: 36932998). Western blot images showed that bands larger than 100kd may be proteins modified by UFM1.

Protocols

Product Specific Protocols
WB protocol for UFM1 antibody 15883-1-APDownload protocol
IHC protocol for UFM1 antibody 15883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
View Article