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  • KD/KO Validated

DNAJB3 Polyclonal antibody

DNAJB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17177-1-AP

Synonyms

DNAJB3, HCG3



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human kidney tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17177-1-AP targets DNAJB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB3 fusion protein Ag10878
Full Name DnaJ (Hsp40) homolog, subfamily B, member 3
Calculated Molecular Weight 145 aa, 17 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC024013
Gene Symbol DNAJB3
Gene ID (NCBI) 414061
RRIDAB_2094420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAJB3 antibody 17177-1-APDownload protocol
IHC protocol for DNAJB3 antibody 17177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

PMID: 26400768

Authors - Mohamed Abu-Farha
mouseWB

Sci Rep

Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.

Authors - Abdoulaye Diane
  • KD Validated
humanWB,IHC

PLoS One

DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise.

Authors - Abubaker Jehad J
mouseWB

Int J Mol Sci

Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice.

Authors - Ryo Mitsugi
humanWB,IHC

Lipids Health Dis

Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.

Authors - Ali Tiss
mouseWB

Sci Rep

DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.

Authors - Abdelilah Arredouani
  • KD Validated