PFKFB1 Polyclonal antibody

PFKFB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21718-1-AP

Synonyms

6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX



Tested Applications

Positive WB detected inHepG2 cells, SMM-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21718-1-AP is PFKFB1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB1 fusion protein Ag16362
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Calculated Molecular Weight 471 aa, 55 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC096079
Gene Symbol PFKFB1
Gene ID (NCBI) 5207
RRIDAB_2878911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PFKFB1 antibody 21718-1-APDownload protocol
IHC protocol for PFKFB1 antibody 21718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols