Histone H3K27me2me3 antibody (mAb) (Clone 7B11)
Host / Isotype
Mouse / IgG
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, IF, WB
CloneNo.
7B11
Cat No : 39435,39535,39537 39535
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ChIP, ChIP-Seq, IF, WB
Applications Validated by Active Motif: ChIP: 1 - 3 ul per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 di/trimethyl Lys27 antibody was validated for ChIP-Seq in the study (see reference). |
| Tested Reactivity | Human, Mouse, Wide Range Predicted |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Modification | Methylated |
| Immunogen | This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3. |
| Full Name | Histone H3K27me2me3 antibody (mAb) (Clone 7B11) |
| Synonyms | histone H3, histone, H3, histone-H3, histoneH3, mAb, monoclonal, H3K27me3, Histone H3 trimethyl Lys27, Histone H3 trimethyl Lysine 27, Histone H3 trimethyl Lys 27, Histone H3 trimethyl K27, Histone H3 trimethyl K 27, Histone H3 tri-methyl Lys27, Histone H3 tri-methyl Lysine 27, Histone H3 tri-methyl Lys 27, Histone H3 tri-methyl K27, Histone H3 tri-methyl K 27, Histone H3 3Me Lys27, Histone H3 3Me Lysine 27, Histone H3 3Me Lys 27, Histone H3 3Me K27, Histone H3 3Me K 27, Histone H3 Me3 Lys27, Histone H3 Me3 Lysine 27, Histone H3 Me3 Lys 27, Histone H3 Me3 K27, Histone H3 Me3 K 27, antibody, antibodies, chip, chromatin immunoprecipitation, H3K27Me3, polycomb, stem, peptest, sample |
| Molecular weight | 17 kDa |
| GenBank accession number | NP_003522 |
| RRID | AB_2793246 |
| Purification Method | Ascites |
| Buffer | Ascites containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39536) of this antibody that was purified by Protein A Chromatography is also available. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.