RBPJ antibody (mAb) (Clone 1F1)

Host / Isotype

Rat / IgG2b

Reactivity

Human, Mouse

Applications

ChIP, EMSA, ICC, IF

CloneNo.

1F1

Cat No : 61505,61506

Synonyms

RBPJ, Recombination Signal Binding Protein For Immunoglobulin Kappa J Region, Suppressor Of Hairless Homolog (Drosophila), IGKJRB1, AOS3, RBPSUH, CBF1, Renal Carcinoma Antigen NY-REN-30, KBF2, CBF-1, SUH, IGKJRB, csl, RBP-J, H-2K Binding Factor-2, RBP-JK, Immunoglobulin Kappa J Region Recombination Signal Binding Protein 1, RBPJK, Recombining Binding Protein Suppressor Of Hairless, RBP-J Kappa, Suppressor Of Hairless Homolog, Recombining Binding Protein Suppressor Of Hairless (Drosophila), J Kappa-Recombination Signal-Binding Protein, RBPJκ, RBPJ1, Supressor of hairless, SUH, transcriptional, Notch, monoclonal, mab, sample

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Validation Data Gallery

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  RBPJ antibody (mAb) tested by ChIP. Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG.

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  RBPJ antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with RBPJ antibody at a 0.5 ug/ml dilution.

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Product Information

• Tested Applications: ChIP, EMSA, ICC, IF

  Applications Validated by Active Motif: ICC/IF: 0.5 ug/ml dilution

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rat / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: This antibody was raised against a full-length recombinant protein corresponding to human RBPJ.
• Full Name: RBPJ antibody (mAb) (Clone 1F1)
• Synonyms: RBPJ, Recombination Signal Binding Protein For Immunoglobulin Kappa J Region, Suppressor Of Hairless Homolog (Drosophila), IGKJRB1, AOS3, RBPSUH, CBF1, Renal Carcinoma Antigen NY-REN-30, KBF2, CBF-1, SUH, IGKJRB, csl, RBP-J, H-2K Binding Factor-2, RBP-JK, Immunoglobulin Kappa J Region Recombination Signal Binding Protein 1, RBPJK, Recombining Binding Protein Suppressor Of Hairless, RBP-J Kappa, Suppressor Of Hairless Homolog, Recombining Binding Protein Suppressor Of Hairless (Drosophila), J Kappa-Recombination Signal-Binding Protein, RBPJκ, RBPJ1, Supressor of hairless, SUH, transcriptional, Notch, monoclonal, mab, sample
• Molecular weight: 68 kDa
• GenBank accession number: NP_056958
• RRID: AB_2614972
• Purification Method: Protein G Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.