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TNF antibody (mAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA

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Cat No : 61601,61602 61601

Synonyms

TNF, Tumor Necrosis Factor, Tumor Necrosis Factor (Superfamily, Member 2), TNFA, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, APC1 Protein, -A1, cachectin, -Alpha, Macrophage-Derived, TNFSF2, Monocyte-Derived, Member 2, Tumor Necrosis Factor-Alpha, Superfamily, Cachectin, cytokine, TNFRSF1a/NFR1, TNFRSF1B/TNFBR, cell death, antibody, antibodies, mab, monoclonal, elisa, bioassay, sample

Validation Data Gallery

  • TNF antibody (mAb) tested by ELISA. Recombinant TNF protein or a negative control protein was conjugated to Luminex microspheres and then incubated in the same well with a serial dilution of TNF antibody (mAb). Biotinylated anti-mouse IgG followed by Streptavidin-PE was used to detect the bound TNF antibody . Mean Fluorescence Intensity (MFI) for each protein was measured in a Luminex 200.

    TNF antibody (mAb) tested by ELISA. Recombinant TNF protein or a negative control protein was conjugated to Luminex microspheres and then incubated in the same well with a serial dilution of TNF antibody (mAb). Biotinylated anti-mouse IgG followed by Streptavidin-PE was used to detect the bound TNF antibody . Mean Fluorescence Intensity (MFI) for each protein was measured in a Luminex 200.

Product Information

Tested Applications ELISA

Applications Validated by Active Motif: ELISA: Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Human TNF.
Full Name TNF antibody (mAb)
Synonyms TNF, Tumor Necrosis Factor, Tumor Necrosis Factor (Superfamily, Member 2), TNFA, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, APC1 Protein, -A1, cachectin, -Alpha, Macrophage-Derived, TNFSF2, Monocyte-Derived, Member 2, Tumor Necrosis Factor-Alpha, Superfamily, Cachectin, cytokine, TNFRSF1a/NFR1, TNFRSF1B/TNFBR, cell death, antibody, antibodies, mab, monoclonal, elisa, bioassay, sample
Molecular weight 17 kDa
GenBank accession numberNP_000585
RRIDAB_2793696
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

TNF (Tumor Necrosis Factor) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.