1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.