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AE2 Polyclonal antibody

AE2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26332-1-AP

Synonyms

AE 2, AE2, Anion exchange protein 2, Anion exchanger 2, BND3L, EPB3L1, HKB3, MPB3L, NBND3, SLC4A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AE2 Polyclonal antibody (26332-1-AP)

    WB analysis using 26332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26332-1-AP (AE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AE2 Polyclonal antibody (26332-1-AP)

    IHC staining of human liver cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AE2 Polyclonal antibody (26332-1-AP)

    IHC staining of human liver cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AE2 Polyclonal antibody (26332-1-AP)

    IHC staining of human lung cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AE2 Polyclonal antibody (26332-1-AP)

    IHC staining of human lung cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26332-1-AP targets AE2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AE2 fusion protein Ag23673
Full Name solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1)
Calculated Molecular Weight 1241 aa, 137 kDa
Observed Molecular Weight 150 and 70 kDa
GenBank Accession NumberBC028601
Gene Symbol SLC4A2
Gene ID (NCBI) 6522
RRIDAB_2880481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC4A2, also named as AE2, encodes a Cl-/HCO3- exchanger. It has been reported that the reduced expression of SLC4A2 found in the liver and peripheral blood mononuclear cells of patients may be associated with the hypermethylation of SLC4A2 promoter regions, which might contribute to the pathogenesis of primary biliary cholangitis (PBC). 26332-1-AP antibody detects 150 kDa and 70 kDa (XP_006716157, 666aa) protein in SDS-PAGE. (PMID: 23341620, 32039364)

Protocols

Product Specific Protocols
WB protocol for AE2 antibody 26332-1-APDownload protocol
IHC protocol for AE2 antibody 26332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
View Article