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AFAP1L2 Polyclonal antibody
AFAP1L2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17183-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse thymus tissue |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
17183-1-AP targets AFAP1L2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AFAP1L2 fusion protein Ag10966 |
| Full Name | actin filament associated protein 1-like 2 |
| Calculated Molecular Weight | 818 aa, 91 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC033212 |
| Gene Symbol | AFAP1L2 |
| Gene ID (NCBI) | 84632 |
| RRID | AB_2226101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AFAP1L2, also named as Actin filament-associated protein 1-like 2 or XB130, is a 818 amino acid protein, which contains two PH domains. AFAP1L2 is detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas. AFAP1L2 localizes in the cytoplasm and may play a role in a signaling cascade by enhancing the kinase activity of SRC. AFAP1L2 promotes thyroid tumor growth, and its expression in these cancer cells may affect cell proliferation and survival by controlling the expression of multiple genes, especially transcription regulators.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AFAP1L2 antibody 17183-1-AP | Download protocol |
| IHC protocol for AFAP1L2 antibody 17183-1-AP | Download protocol |
| IP protocol for AFAP1L2 antibody 17183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors. | ||
Carcinogenesis XB130 deficiency enhances carcinogen-induced skin tumorigenesis.
| ||
Oncol Lett The 3'‑untranslated region of XB130 regulates its mRNA stability and translational efficiency in non‑small cell lung cancer cells | ||
Cell Rep Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tamara (Verified Customer) (12-02-2020) | antibody works very well in detecting endogenous Afap1l2 protein in mouse prostate lysates
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