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AMZ1 Polyclonal antibody

AMZ1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24484-1-AP

Synonyms

AMZ1, Archaemetzincin 1, KIAA1950

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    WB analysis of mouse liver using 24484-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    WB analysis of rat brain using 24484-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    WB analysis of mouse brain using 24484-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    WB analysis of mouse liver using 24484-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    WB analysis of mouse liver using 24484-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    IHC staining of human heart using 24484-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AMZ1 Polyclonal antibody (24484-1-AP)

    IHC staining of human heart using 24484-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AMZ1 Polyclonal antibody (24484-1-AP)

    IF Staining of HepG2 using 24484-1-AP

    Immunofluorescent analysis of HepG2 cells using 24484-1-AP (AMZ1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24484-1-AP is AMZ1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMZ1 fusion protein Ag20248
Full Name archaelysin family metallopeptidase 1
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 33 kDa, 28 kDa
GenBank Accession NumberBC146753
Gene Symbol AMZ1
Gene ID (NCBI) 155185
RRIDAB_2879565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMZ1, also named as KIAA1950, is a new human Zinc metalloproteases. It exhibits aminopeptidase activity against neurogranin in vitro. AMZ1 does not hydrolyze angiotensin-2. It is mainly detected in liver and heart. AMZ1 has some isoforms with MW 55 kDa, 33 kDa, 30 kDa and 28 kDa.

Protocols

Product Specific Protocols
WB protocol for AMZ1 antibody 24484-1-APDownload protocol
IHC protocol for AMZ1 antibody 24484-1-APDownload protocol
IF protocol for AMZ1 antibody 24484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols