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ARD1A Polyclonal antibody

ARD1A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14803-1-AP

Synonyms

ARD1, ARD1A, NAA10, Naa10p, TE2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARD1A Polyclonal antibody (14803-1-AP)

    WB analysis using 14803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14803-1-AP (ARD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ARD1A Polyclonal antibody (14803-1-AP)

    WB analysis of mouse spleen using 14803-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14803-1-AP (ARD1A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ARD1A Polyclonal antibody (14803-1-AP)

    IP experiment of MCF-7 using 14803-1-AP

    IP result of anti-ARD1A (IP:14803-1-AP, 4ug; Detection:14803-1-AP 1:500) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARD1A Polyclonal antibody (14803-1-AP)

    IHC staining of human breast cancer using 14803-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14803-1-AP (ARD1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARD1A Polyclonal antibody (14803-1-AP)

    IHC staining of human breast cancer using 14803-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14803-1-AP (ARD1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ARD1A Polyclonal antibody (14803-1-AP)

    IF Staining of MCF-7 using 14803-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ARD1A antibody 14803-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHL-60 cells, mouse spleen tissue, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14803-1-AP targets ARD1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARD1A fusion protein Ag6519
Full Name ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC000308
Gene Symbol ARD1A
Gene ID (NCBI) 8260
RRIDAB_1557262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARD1A antibody 14803-1-APDownload protocol
IHC protocol for ARD1A antibody 14803-1-APDownload protocol
IF protocol for ARD1A antibody 14803-1-APDownload protocol
IP protocol for ARD1A antibody 14803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Elife

Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway.

Authors - Hyae Yon Kweon
View Article
YeastWB

Yeast

Proteomic and genomic characterization of a yeast model for Ogden syndrome.

Authors - Max J Dörfel
View Article

Methods Enzymol

Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities

Authors - Jürgen Eirich
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Zehua (Verified Customer) (07-09-2018)

Samples from Proteintech. Works very well for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF-7