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ATP5E Polyclonal antibody

ATP5E Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15408-1-AP

Synonyms

ATP5E, ATPase subunit epsilon, ATPE

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5E Polyclonal antibody (15408-1-AP)

    WB analysis using 15408-1-AP

    Boiled and 37℃ incubated mouse heart tissue were subjected to SDS PAGE followed by western blot with 15408-1-AP (ATP5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5E Polyclonal antibody (15408-1-AP)

    IF Staining of HeLa using 15408-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5E Polyclonal antibody (15408-1-AP)

    IF Staining of HeLa using 15408-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue, 37℃ incubated mouse heart tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15408-1-AP targets ATP5E in WB, IF, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5E fusion protein Ag7672
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Calculated Molecular Weight 6 kDa
Observed Molecular Weight6 kDa
GenBank Accession NumberBC001690
Gene Symbol ATP5E
Gene ID (NCBI) 514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP synthase epsilon subunit (ATP5E) is an important subunit of ATP synthase, which is located in the stalk region of the F1 sector. F1-ATPase is the catalytic portion of mitochondrial ATP synthase, which produces ATP from ADP and inorganic phosphate (Pi). ATP5E is widely expressed in tissues. The calculated molecular weight of ATP5E is 6 kDa (PMID: 36625260).

Protocols

Product Specific Protocols
WB protocol for ATP5E antibody 15408-1-APDownload protocol
IF protocol for ATP5E antibody 15408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols