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MCPH/BRIT1 Polyclonal antibody
MCPH/BRIT1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11962-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, MDA-MB-453s cells, mouse ovary tissue |
| Positive IHC detected in | human prostate cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
11962-1-AP targets MCPH/BRIT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCPH/BRIT1 fusion protein Ag2567 |
| Full Name | microcephalin 1 |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 66 kDa, 100 kDa |
| GenBank Accession Number | BC030702 |
| Gene Symbol | MCPH1 |
| Gene ID (NCBI) | 79648 |
| RRID | AB_2142989 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCPH/BRIT1 antibody 11962-1-AP | Download protocol |
| IHC protocol for MCPH/BRIT1 antibody 11962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1.
| ||
Stem Cell Res Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology.
| ||
FEBS J Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1.
| ||
Genes (Basel) MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Paul (Verified Customer) (11-10-2022) | I compared several commercial antibodies for MCPH1 and this was the only one that was able to detect both isoforms in Western blotting. Importantly, these bands disappeared after treatment of cells with MCPH1 siRNA.
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