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BXDC2 Polyclonal antibody

BXDC2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17295-1-AP

Synonyms

BRIX, brix domain containing 2, BRIX1, BXDC2

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using BXDC2 Polyclonal antibody (17295-1-AP)

    WB analysis of human liver using 17295-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BXDC2 Polyclonal antibody (17295-1-AP)

    WB analysis of HEK-293 using 17295-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BXDC2 Polyclonal antibody (17295-1-AP)

    WB analysis of HEK-293 using 17295-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BXDC2 Polyclonal antibody (17295-1-AP)

    WB analysis of HEK-293 using 17295-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BXDC2 Polyclonal antibody (17295-1-AP)

    WB analysis of HeLa using 17295-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BXDC2 Polyclonal antibody (17295-1-AP)

    WB analysis of HEK-293 using 17295-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BXDC2 Polyclonal antibody (17295-1-AP)

    IP experiment of HEK-293 using 17295-1-AP

    IP result of anti-BXDC2 (IP:17295-1-AP, 4ug; Detection:17295-1-AP 1:700) with HEK-293 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using BXDC2 Polyclonal antibody (17295-1-AP)

    IHC staining of human pancreas using 17295-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17295-1-AP (BXDC2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using BXDC2 Polyclonal antibody (17295-1-AP)

    IHC staining of human pancreas using 17295-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17295-1-AP (BXDC2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BXDC2 Polyclonal antibody (17295-1-AP)

    IF Staining of HepG2 using 17295-1-AP

    Immunofluorescent analysis of HepG2 cells, using BXDC2 antibody 17295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman liver tissue, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17295-1-AP targets BXDC2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BXDC2 fusion protein Ag11174
Full Name brix domain containing 2
Calculated Molecular Weight 353 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC001546
Gene Symbol BXDC2
Gene ID (NCBI) 55299
RRIDAB_1939420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BXDC2 antibody 17295-1-APDownload protocol
IHC protocol for BXDC2 antibody 17295-1-APDownload protocol
IF protocol for BXDC2 antibody 17295-1-APDownload protocol
IP protocol for BXDC2 antibody 17295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols