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C18orf21 Polyclonal antibody

C18orf21 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24977-1-AP

Synonyms

C18orf21, HsT3108, PNAS 124, PNAS 131, UPF0711 protein C18orf21, XTP13

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using C18orf21 Polyclonal antibody (24977-1-AP)

    WB analysis of COLO 320 using 24977-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human colon cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human colon cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using C18orf21 Polyclonal antibody (24977-1-AP)

    IF Staining of COLO 320 using 24977-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24977-1-AP is C18orf21 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C18orf21 fusion protein Ag21782
Full Name chromosome 18 open reading frame 21
Calculated Molecular Weight 220 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC119766
Gene Symbol C18orf21
Gene ID (NCBI) 83608
RRIDAB_2879829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for C18orf21 antibody 24977-1-APDownload protocol
IHC protocol for C18orf21 antibody 24977-1-APDownload protocol
IF protocol for C18orf21 antibody 24977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols