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C21orf45 Polyclonal antibody

C21orf45 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 25832-1-AP

Synonyms

B28, C21orf45, C21orf46, FAPP1 associated protein 1, FASP1, MIS18A, MIS18alpha, Protein Mis18 alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C21orf45 Polyclonal antibody (25832-1-AP)

    WB analysis of HeLa using 25832-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25832-1-AP (C21orf45 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C21orf45 Polyclonal antibody (25832-1-AP)

    IF Staining of HeLa using 25832-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25832-1-AP (C21orf45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25832-1-AP is C21orf45 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf45 fusion protein Ag22922
Full Name chromosome 21 open reading frame 45
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC042917
Gene Symbol C21orf45
Gene ID (NCBI) 54069
RRIDAB_2880259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer(~52 kDa) and heterodimer(~105 kDa) forms.

Protocols

Product Specific Protocols
WB protocol for C21orf45 antibody 25832-1-APDownload protocol
IF protocol for C21orf45 antibody 25832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF, WB

Br J Pharmacol

Nuclear translocation of ATG5 induces DNA mismatch repair deficiency (MMR-D)/microsatellite instability (MSI) via interacting to Mis18α in colorectal cancer.

Authors - Shi-Yue Sun
View Article