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  • Featured Product
  • KD/KO Validated

Caldesmon Polyclonal antibody

Caldesmon Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20887-1-AP

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Caldesmon Polyclonal antibody (20887-1-AP)

    WB analysis of HeLa using 20887-1-AP

    WB result of Caldesmon antibody (20887-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caldesmon transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Caldesmon Polyclonal antibody (20887-1-AP)

    WB analysis using 20887-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Caldesmon Polyclonal antibody (20887-1-AP)

    IHC staining of human colon using 20887-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Caldesmon Polyclonal antibody (20887-1-AP)

    IHC staining of mouse heart using 20887-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Caldesmon Polyclonal antibody (20887-1-AP)

    IF Staining of mouse heart using 20887-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Caldesmon Polyclonal antibody (20887-1-AP)

    IF Staining of HeLa using 20887-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Caldesmon Polyclonal antibody (20887-1-AP)

    IF Staining of mouse heart using 20887-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Caldesmon antibody (20887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Caldesmon Polyclonal antibody (20887-1-AP)

    FC experiment of HeLa using 20887-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Caldesmon (20887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inA2780 cells, HeLa cells, HT-1080 cells, MDA-MB-231 cells, PC-3 cells, SKOV-3 cells
Positive IHC detected inhuman colon tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20887-1-AP targets Caldesmon in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caldesmon fusion protein Ag13676
Full Name caldesmon 1
Calculated Molecular Weight 538 aa, 63 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC040354
Gene Symbol CALD1
Gene ID (NCBI) 800
RRIDAB_10792408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).

Protocols

Product Specific Protocols
WB protocol for Caldesmon antibody 20887-1-APDownload protocol
IHC protocol for Caldesmon antibody 20887-1-APDownload protocol
IF protocol for Caldesmon antibody 20887-1-APDownload protocol
FC protocol for Caldesmon antibody 20887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Am Heart Assoc

Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.

Authors - Jiaqi Tang
View Article
mouseWB

Sci Rep

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Authors - Dongli Liang
View Article
humanWB,IF

Front Physiol

PMID: 30108509

Authors - Iddrisu B Yabasin
View Article
humanWB

Front Med (Lausanne)

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Authors - Fang He
View Article
mouse

bioRxiv

IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells

Authors - Victoria A Wong
View Article
humanWB

Cancers (Basel)

AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma

Authors - Jiakang Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Gaurav (Verified Customer) (01-22-2024)

Excellent antibody. Results are better than expected. Works well in Co-IP as well as in western blotting

  • Applications: Western Blot, Immunoprecipitation, Cell culture
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293, A7R5, HTR8, Liver, Placenta, Aorta
Caldesmon Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in HEK293, A7R5, HTR8, Liver, Placenta, Aorta (Cat no:20887-1-AP)