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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CAPG Monoclonal antibody

CAPG Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A5B2

Cat no : CL488-66277

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CAPG Monoclonal anti (CL488-66277)

    IF Staining of HeLa using CL488-66277

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66277 (CAPG antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66277 targets CAPG in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPG fusion protein Ag24676
Full Name capping protein (actin filament), gelsolin-like
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC000728
Gene Symbol CAPG
Gene ID (NCBI) 822
RRIDAB_2883300
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CAPG antibody CL488-66277Download protocol
Standard Protocols
Click here to view our Standard Protocols