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CCDC153 Polyclonal antibody

CCDC153 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21390-1-AP

Synonyms

CCDC153

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of COLO 320 using 21390-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of HepG2 using 21390-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of L02 using 21390-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of human brain using 21390-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using CCDC153 Polyclonal antibody (21390-1-AP)

    IHC staining of human prostate hyperplasia using 21390-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21390-1-AP (CCDC153 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using CCDC153 Polyclonal antibody (21390-1-AP)

    IHC staining of human prostate hyperplasia using 21390-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21390-1-AP (CCDC153 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    IF Staining of HepG2 using 21390-1-AP

    Immunofluorescent analysis of HepG2 cells, using CCDC153 antibody 21390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC153 Polyclonal antibody (21390-1-AP)

    IF Staining of HeLa using 21390-1-AP

    Immunofluorescent analysis of Hela cells, using CCDC153 antibody 21390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells, human brain tissue, L02 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21390-1-AP is CCDC153 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC153 fusion protein Ag16044
Full Name coiled-coil domain containing 153
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC101443
Gene Symbol CCDC153
Gene ID (NCBI) 283152
RRIDAB_10858621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC153's properties have yet to be elucidated. However, research suggests that it may be a neuronal subtype marker (PMID: 28166221). It has a molecular weight of 24 KDa and is often observed on a Western blot as a dimer.

Protocols

Product Specific Protocols
WB protocol for CCDC153 antibody 21390-1-APDownload protocol
IHC protocol for CCDC153 antibody 21390-1-APDownload protocol
IF protocol for CCDC153 antibody 21390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols